Low- and high-voltage electron microscopy of a human neuroblastoma in long-term organ culture.
نویسنده
چکیده
A human neuroblastoma was maintained in a continuing organ culture, in which three-dimensional organization and cell proliferation occurred together. Electron micrographs of the cells showed a typical immature appearance: scant cytoplasm, numerous free ribosomes, poorly developed granular endoplasmic reticulum, and a large nucleus and prominent nucleolus. Cellular processes with microtubules were found to be present. Thus, the fine structure of the neuroblastoma in organotypic culture was similar to that of the original tumor and to ganglion cells at early stages of embryonic development.
منابع مشابه
Revolutionizing Tissue Engineering through Mirroring Cell Niche and Application of Natural Compounds
onsidering the high prevalence of severe organ failures due to the cancer, congenital anomaly, or trauma, and the consequent needs for tissue transplantation, deficiencies in tissues and organs are a huge challenge for regenerative medicine at the moment. More than 40 years have passed since the term ‘tissue engineering’ was created as a new th...
متن کاملFacile synthesis of high magnetization long term stable bimetallic FeCo nanoparticles
In this study, we reported a facile synthesis of bimetallic FeCo nanoparticles (Fe-Co NPs) by FeSO4.7H2O and Co(Ac)2.4H2O in the presence of sodium borohydride and 2-thiotic acid. The structure and morphology of the nanoparticles were characterized by X-Ray Diffraction (XRD), Energy-dispersive X-ray spectroscopy (EDS), and Transmission Electron Micros...
متن کاملAn Unfavorable Metastatic Site at Initial Diagnosis in a 2-year-old Boy with Neuroblastoma
Neuroblastoma (NB) is considered one of the malignant tumors of the nervous system which originates from primordial neural crest cell and is known as the most common extra-cranial solid tumor in childhood. This tumor which is often intra-abdominal, metastases in various sites including the skull, long bones, liver, bone marrow and lymph nodes, but lung metastasis at initial diagnosis is rare (0...
متن کاملVoltammetric Investigation of Thioridazine Using a Carbon Nanocomposite Electrode in Human Biological Samples
An electrochemical method employing a carbon nanocomposite electrode was developed to measure thioridazine. The electrode has been designed by incorporation of montmorillonite nanoclay into the carbon ionic liquid electrode. Surface morphology of the electrodes was done using scanning electron microscopy. Adsorptive stripping voltammetry was applied as a very sensitive analytical method for...
متن کاملLong-term organ culture of normal human bladder.
Normal adult human bladder obtained at cystoscopy has been maintained in long-term organ culture. Several media were tested for their ability to maintain viability and normal tissue morphology. The optimum medium was Ham's F-12 nutrient mixture, supplemented with 10% fetal calf serum, hydrocortisone (1 microgram/ml), and FeSO4, (0.45 microgram/ml). During the first 28 days in vitro, epithelial ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Cancer research
دوره 34 3 شماره
صفحات -
تاریخ انتشار 1974